Selection and Characterization of a Bacillus Strain for Potential Application in Industrial Production of White Button Mushroom (Agaricus bisporus)

White button mushroom—Agaricus bisporus (J.E.Lange) Imbach—is among the most popular cultivated mushrooms worldwide. The serious challenge in industrial mushroom production is green mold disease caused by Trichoderma species. Our aim was to isolate and examine bacterial strains from casing material for their potential use as biocontrol agents. Twenty-seven were isolated tested against pathogens of white mushroom. Bacillus velezensis strain SZMC 25431 selected further examination under simulated Agaricus cultivation conditions T. aggressivum 23834 a 1200-L Fitotron SGC120 standard plant growth chamber. results showed that treatment effective pathogen all cases, but best achieved at an application concentration 105 cells mL−1. Industrial-scale experiments also carried out growing houses with bearing surface 480 m2: suspension mixed water tanks applied daily irrigation. suggest may even increase crop yield A. bisporus. Based on our results, we concluded B. potentially be used biological integrated cultivation.